Genome editing, considering through conventional breeding

Is Genome Editing Techniques the New Challenge for Plant Breeding?

Plant breeding is the “art and science” of the genetic improvement of crops to produce new varieties that had a large impact on crop yield and food production. Genetic variation is the engine that propels breeding to meet future challenges and this variation is due to intraand inter-specific recombination. The recombinant DNA technology marked the beginning of a new era, indeed conventional pla...

Advances in genome editing for improved animal breeding: A review

Since centuries, the traits for production and disease resistance are being targeted while improving the genetic merit of domestic animals, using conventional breeding programs such as inbreeding, outbreeding, or introduction of marker-assisted selection. The arrival of new scientific concepts, such as cloning and genome engineering, has added a new and promising research dimension to the exist...

Antiviral Defenses in Plants through Genome Editing

Plant-virus interactions based-studies have contributed to increase our understanding on plant resistance mechanisms, providing new tools for crop improvement. In the last two decades, RNA interference, a post-transcriptional gene silencing approach, has been used to induce antiviral defenses in plants with the help of genetic engineering technologies. More recently, the new genome editing syst...

Genome Editing



Optimization of genome editing through CRISPR-Cas9 engineering

CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 base pairs upstream of a PAM (Protospacer Adjacent Motif). The broken DNA ends ...